Biolegend, 516407, Biotin anti-mouse IL-22 Antibody
50microg
Catalog Number: 516407
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| Verified Reactivity | Mouse |
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| Antibody Type | Polyclonal |
| Host Species | Goat |
| Immunogen | Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | ELISA Detection - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.2-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-22 recombinant protein ranging from 250 to 3.9 pg/ml are recommended. |
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| Application Notes |
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| ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard. In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively. Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations. Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 & 436308) is specially developed and recommended. Note: The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization.1 |
| Application References(PubMed link indicates BioLegend citation) | Veldhoen M, et al. 2009. J. Exp. Med. 206:43. |
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| Product Citations | Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed |
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| RRID | AB_2561414 (BioLegend Cat. No. 516407) |
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| Structure | A cytokine structurally related to IL-10 with a predicted molecular weight of 20 kD. |
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| Distribution | Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells. |
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| Function | Acts on nonhematopoietic tissue cells, such as epithelial cells and keratinocytes, involved in inflammatory processes, mucosal immunity, and wound healing. |
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| Cell Type | NK cells, Th1, Th17, Th2 |
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| Biology Area | Immunology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Martin B, et al. 2009. Immunity 31:321. 2. Liang SC, et al. 2006. J. Exp. Med. 203:2271. 3. Veldhoen M, et al. 2008. J. Exp. Med. 206:43. 4. Zheng Y, et al. 2007. Nature 445:648. 5. Dumoutier L, et al. 2000. J. Immunol. 164:1814. 6. Wolk K, et al. 2007. J. Immunol. 178:5973. |
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| Gene ID | 50929 |
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| UniProt | View information about IL-22 on UniProt.org |
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| Clone | Poly5164 |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF |
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| Isotype | Goat Polyclonal IgG |
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