Biolegend, 516503, Biotin anti-mouse IL-33 Antibody
50microg
Catalog Number: 516503
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| Verified Reactivity | Mouse, Rat |
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| Antibody Type | Polyclonal |
| Host Species | Goat |
| Immunogen | Mouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide. |
| Preparation | The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | ELISA Detection - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000 to 15.6 pg/ml are recommended. |
| Application Notes | ELISA Detection: The biotinylated Poly5165 antibody is useful as a detection antibody in a sandwich ELISA assay, when used in conjunction with purified Poly5165 (Cat. No. 516501) antibody as the capture antibody and recombinant mouse IL-33 (Cat. No. 580509) as the standard. |
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| Application References(PubMed link indicates BioLegend citation) | Gao X, et al. 2015. J Immunol. 194:438. PubMed |
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| Product Citations | Gao X, et al. 2015. J Immunol. 194:438. PubMed |
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| RRID | AB_2561415 (BioLegend Cat. No. 516503) |
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| Distribution | High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues. |
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| Function | IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-a and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes. |
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| Ligand/Receptor | IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein). |
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| Cell Type | Epithelial cells, Fibroblasts |
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| Biology Area | Immunology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Schmitz J, et al. Immunity 2005 23:479 2. Barksby HE, et al. C Exp Immunol 2007 149:217 3. Arend WP, et al. ImmRev 2008 223:20 4. Suzukawa M, et al. J. Immunol. 2008 181:5981 5. Moussio C, et al. PlosOne 2008 10:e3331. |
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| Gene ID | 77125 |
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| UniProt | View information about IL-33 on UniProt.org |
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| Clone | Poly5165 |
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| Regulatory Status | RUO |
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| Other Names | Il-1f11, Nuclear factor from high endothelial venules (NF-HEV) |
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| Isotype | Goat Polyclonal Ig |
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