Biolegend, 525904, Biotin anti-mouse IL-27 Antibody
500microg
Catalog Number: 525904
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant mouse IL-27 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | ELISA Detection - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.125 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance. |
| Application Notes | ELISA Detection: To measure mouse IL-27, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified MM27-7B1 antibody (Cat. No. 516902) as the capture antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.Note: For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707 & 438708) are specially developed and recommended. |
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| RRID | AB_2563423 (BioLegend Cat. No. 525903) AB_2563424 (BioLegend Cat. No. 525904) |
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| Structure | A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein). |
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| Distribution | Produced by antigen-presenting cells, including macrophages and dendritic cells. |
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| Function | Drives rapid clonal expansion of naïve CD4+ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy. |
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| Interaction | T cells and NK cells. |
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| Ligand/Receptor | IL-27R (TCCR) (WSX-1). |
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| Cell Type | Antigen-presenting cells, Macrophages, Dendritic cells, B cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Salcedo R, et al. 2004. J. Immunol. 173:7170. 2. Owaki T, et al. 2005. J. Immunol. 175:2191. 3. Pflanz S, et al. 2002. Immunity 16:779. 4. Diveu C, et al. 2009. J. Immunol. 182:5748. 5. Morishima N, et al. 2005. J. Immunol. 175:1686. 6. Liu J, et al. 2007. J. Exp. Med. 204:141. |
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| Gene ID | 246779 |
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| UniProt | View information about IL-27 on UniProt.org |
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| Clone | B02P6E6 |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-27 p28, IL-27-A, IL-27 subunit alpha |
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| Isotype | Rat IgG2a, κ |
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