Biolegend, 551224, Cell-Vive™ GMP Recombinant Human Betacellulin (carrier-free)
50microg
Catalog Number: 551224
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| Source | Human Betacellulin, amino acids (Asp32-Tyr111) (Accession# NM_001720.1), was expressed in E. coli. |
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| Molecular Mass | The 80 amino acid recombinant protein has a predicted molecular mass of approximately 8.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 13 -15 kD by SDS-PAGE. The predicted N-terminal amino acid is Asp. |
| N-terminalSequence Analysis | Asp-Gly-Asn-Ser-Thr-Arg-Ser-Pro-Glu-Thr |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | Protein was lyophilized from 0.1 µm filtered solution containing PBS, pH 7.2 |
| Endotoxin Level | Less than or equal to 0.1 EU per µg of protein as determined by LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/µg by ELISA |
| Concentration | 50 µg size is lyophilized |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile PBS. Before reconstitution, make sure sterile PBS and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile PBS) to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/ thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months. |
| Activity | Recombinant human betacellulin induces the proliferation of murine BALB/3T3 cell line in a dose dependent manner. The ED50 for this effect is 0.01 – 0.1 ng/mL. |
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| Application | Bioassay |
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| Application Notes | This product is reactive with human and mouse. Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Monomer |
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| Distribution | Widely expressed in most tissues and various body fluids, including milk. Expressed in smooth muscle, endothelial cells, mesenchymal stem cells. Highly expressed in pancreas. |
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| Function | Betacellulin induces proliferation of beta cells, pancreatic endocrine precursor cells, and neural stem cells. Betacellulin, amphiregulin, and epiregulin stimulate oocyte maturation and cumulus expansion and its expression is induced by LH. |
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| Interaction | Pancreas endocrine precursor cells, beta cells, fetal pancreatic epithelial cell line, smooth muscle cells, and neuronal stem cells. |
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| Ligand/Receptor | ErbB-1 and ErbB-4 homodimers and heterodimeric ErbB receptors. |
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| Bioactivity | Human betacellulin induces proliferation of murine BALB/3T3 cells. |
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| Antigen References | Sasada R, et al.1993. Biochem Biophys Res Commun. 190:1173-9. Dunbar AJ & Goddard C. 2000. Int J Biochem. Cell Biol. 32:805-15. Sahin U, et al. 2004. J Cell Biol. 164:769-79. O-charoenrat P, et al. 2004. Int J Cancer. 111:174-83. Hsieh M, et al. 2009. Semin Reprod Med. 27:52-61. Stoeck A, et al. 2010. Int J Mol Sci. 123:2319. Oh YS, et al. 2011. PLoS One. 6:e23894. Gomez-Gaviro MV, et al. 2012. Proc Natl Acad Sci USA. 109:1317-22. Dahlhoff M, et al. 2014. Semin. Cell Dev. Biol. 28:42-8. |
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| Gene ID | 685 |
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| UniProt | View information about Betacellulin on UniProt.org |
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| Other Names | BTC |
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