Biolegend, 560701, Recombinant Human IL-5
2microg
Catalog Number: 560701
Send an RFQ.
Category
Loading…
| Source | E. coli, approximately 26 kD |
|---|---|
| Formulation | Sterile-filtered through a 0.2 micron filter. Lyophilized with no additives. |
| Endotoxin Level | Less than 0.1 ng per µg of protein. |
| Preparation | Centrifuge vial prior to opening. Reconstitute in 100 µl sterile water for a concentration of 20 µg/ml and mix well. This solution can be diluted into other aqueous buffers containing a carrier protein such as 1% BSA or HSA or 10% FBS. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer with carrier protein. After reconstitution, the cytokine can be stored at -20°C to -70°C for up to three months. |
| Storage & Handling | Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles. |
| Application | ELISA, Bioassay |
| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. Please see antibody product data sheets and recommended protocols. It is recommended that the reagent be titrated for optimal performance for each application. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes | ELISA: This IL-5 protein is useful as a standard for a human IL-5 sandwich ELISA, using unlabeled JES1-39D10 antibody (catalog #500902) for capture and biotinylated JES1-5A10 antibody (catalog #501002) for detection. Ligand Blocking: This IL-5 protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining. Bioassay: This IL-5 protein is biologically active, and can be used for in vitro assays. |
|---|
| Product Citations | Zhang C, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed |
|---|
| Structure | Cytokine; dimer |
|---|
| Bioactivity | Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes |
|---|
| Cell Sources | Mast cells, T cells, bone marrow stromal cells |
|---|
| Cell Targets | Eosinophils |
|---|
| Receptors | Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR |
|---|
| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors |
|---|
| Biology Area | Cell Biology, Immunology, Stem Cells |
|---|
| Molecular Family | Cytokines/Chemokines |
|---|
| Antigen References | 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107. 3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu, K. 1991. Microbiol. Immun. 35:593. |
|---|
| Regulation | Upregulated by IL-2, platelet activating factor; downregulated by TGF-β |
|---|
| Gene ID | 3567 |
|---|
| UniProt | View information about IL-5 on UniProt.org |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1) |
|---|
