Biolegend, 566704, Biotinylated Recombinant Human SLAMF6-Fc Chimera (carrier-free)
25microg
Catalog Number: 566704
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| Source | Human SLAMF6 (NTB-A), amino acid (Leu28-Lys225) (Accession: # Q96DU3.3), with a linker (GS), a C-terminal human IgG (Pro100-Lys330) an Avi-tag, was expressed in CHO cells. Human SLAMF6-Avi tag was site-specifically biotinylated by enzyme BirA. |
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| Molecular Mass | The 446 amino acid recombinant protein has a predicted molecular mass of approximately 50.23 kD. The DTT-reduced and non-reduced protein migrates at approximately 62 – 80 kD and 115 – 140 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS. |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | When recombinant human SLAMF6-Fc (Cat. No. 784606) is immobilized at 1 µ/mL, biotinylated recombinant human SLAMF6 binds in a dose-dependent manner with ED50 of 1 – 6 µg/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | CD2 subfamily |
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| Distribution | Expressed in resting and activated NK cells, T cells, B cells; plasma membrane |
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| Function | CD28 independent T cell co-stimulation, induction of NK cell cytotoxicity, regulation of B cell tolerance; plays an important role in CD4+ T cell responses, innate immunity to bacteria and parasites, and NK cell function |
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| Interaction | SAP/ SH2D1A, EAT-2/ SH2D1B, Src kinase |
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| Ligand/Receptor | SLAMF6 (self-ligand) |
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| Bioactivity | Biotinylated recombinant human SLAMF6-Fc binds to immobilized recombinant human SLAMF6-Fc in a dose-dependent manner using a binding assay. |
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| Cell Type | B cells, Dendritic cells, Granulocytes, Macrophages, NK cells, T cells, Thymocytes |
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| Biology Area | Adaptive Immunity, Cell Biology, Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Immune Checkpoint Receptors, Soluble Receptors |
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| Antigen References | Bottino C, et al. 2001. J ExpMed. 194:235. Eissmann P, et al. 2006. J Immunol. 177:3170-7. Chatterjee M, et al. 2012. J Immunol. 188:1206-12. Howie D, et al. 2005. J Immunol. 174:5931-5. Valdez PA, et al. 2004. J Biol Chem. 279:18662-9. Kumar KR, et al. 2006. Science. 312:1665-9. Flaig RM, et al. 2004. J. Immunol. 172:6524-7. Wang N, et al. 2016. Clin Immunol. 173:19-26. |
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| Gene ID | 114836 |
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| UniProt | View information about SLAMF6 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | SLAMF6, SLAM family member 6, Activating NK receptor, NK-T-Bantigen, NTB-A |
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