Biolegend, 567004, Biotinylated Recombinant Human CD160 (carrier-free)
25microg
Catalog Number: 567004
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| Source | Human CD160, amino acid (Ile27-Ser159) (Accession: # O95971) with a C-terminus 6His and Avi-tag was expressed in CHO cells. Human CD160-Avi tag was site-specifically biotinylated by enzyme BirA. |
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| Molecular Mass | The 169 amino acid recombinant protein has a predicted molecular mass of approximately 18.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 28 – 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Ile. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.2 µm filtered protein solution is in PBS, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per µg of protein as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick the spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | Biotinylated recombinant human CD160 binds to immobilized recombinant human HVEM (Cat. No. 596504) in a dose-dependent manner using a functional ELISA. The binding was detected with HRP Avidin (Cat. No. 405103). The ED50 for this effect is 50 – 250 ng/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomer |
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| Distribution | CD56dim CD16+ NK cells, NKT-cells, γδ T-cells, cytotoxic CD8+ T-cells, a small fraction of CD4+ T-cells, all intraepithelial lymphocytes, and epithelial cells |
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| Function | CD160 binds to MHC class I molecules and enhances NK and T-cell cytolytic activity. CD160 interacts with HVEM (TNFRSF14) and inhibits the proliferation and the cytokine production of T cells. |
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| Interaction | Antigen presenting cell, macrophages, DC, T and B cells |
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| Ligand/Receptor | HLA-G1, HLA-C, and TNFRSF14 |
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| Bioactivity | Biotinylated recombinant human CD160 binds to immobilized recombinant human HVEM in a dose-dependent manner. |
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| Cell Type | Endothelial cells, NK cells, NKT cells |
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| Biology Area | Cell Proliferation and Viability, Costimulatory Molecules, Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Innate Immune Signaling |
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| Antigen References | Mauri DN, et al. 1998. Immunity. 8:21-30. Barakonyi A, et al. 2004. J Immunol. 173:5349-54. Cai G, et al. 2008. Nat Immunol. 9:176-85. Giustiniani J, et al. 2009. J. Immunol. 182:63-71. Šedý JR, et al. 2013. J Immunol. 191:828-36. El-Far M, et al. 2014. J Transl Med. 12:217. Tu TC, et al. 2015. J. Exp Med. 212:415-29. Muscate F, et al. 2018. Front Immunol. 9:2611. |
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| Gene ID | 11126 |
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| UniProt | View information about CD160 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CD160 Antigen, Natural Killer Cell Receptor BY55, BY55, CD160Transmembrane Isoform, CD160-Delta Ig, NK28, NK1 |
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