Biolegend, 568204, Biotinylated Recombinant Human Nectin3-Fc Chimera (carrier-free)
25microg
Catalog Number: 568204
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| Source | Biotinylated recombinant human Nectin-3, amino acid Gly58-Asp400 (Accession No. Q9NQS3) was expressed in 293E cells. The C-terminal includes human IgG1-Fc-Avi tag. |
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| Molecular Mass | The 603 amino acid recombinant protein has a predicted molecular mass of approximately 66.7 kD. The DTT-reduced and non-reduced proteins migrates at approximately 70 - 90 kD and 140 - 200 kD by SDS-PAGE, respectively. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH7.2. |
| Endotoxin Level | Less than 0.1 EU per µg of protein as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Activity | Immobilized recombinant human Nectin-1 binds to Biotinylated Recombinant Human Nectin-3 in a dose-dependent manner. The ED50 for this effect is 1.4 – 8.4 ng/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Dimer |
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| Distribution | Epithelial cells, granule cells, APC, cancer cells, T-cells, and neuronal cells |
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| Function | Adhesion, Ocular development involving the ciliary body |
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| Interaction | T-lymphocytes, NK cell, epithelial cells |
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| Ligand/Receptor | Nectin-1, -2, and -3, TIGIT, and CD155 |
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| Bioactivity | Measured by its ability to bind recombinant human Nectin-1 |
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| Cell Type | Antigen-presenting cells, Endothelial cells, Epithelial cells, Neurons, NK cells, T cells |
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| Biology Area | Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules, Innate Immune Signaling |
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| Antigen References | Takai, Y. and H. Nakanishi. 2003. J Cell Sci. 116:17-27. Takai Y, et al. 2008. Annual Review of Cell and Developmental Biology. 24:309-42. De Agustín-Durán D, et al. 2021. Cells. 10:118. Satoh-Horikawa K, et al. 2000. J Biol Chem. 275:10291-9. Inagaki M, et al. 2005. Development. 132:1525-37. Inagaki M, et al. 2006. Genes Cells. 11:1125-32. |
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| Gene ID | 25945 |
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| UniProt | View information about Nectin3 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Nectin3, Nectin-3, PVRL3, PVRR3, CD113, CD113 antigen, nectin 3, poliovirus receptor-related 3, poliovirus receptor-related protein3, PPR3, PRR3CD113 |
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