Biolegend, 568206, Biotinylated Recombinant Human Nectin3-Fc Chimera (carrier-free)
100microg
Catalog Number: 568206
Send an RFQ.
Category
Loading…
| Source | Biotinylated recombinant human Nectin-3, amino acid Gly58-Asp400 (Accession No. Q9NQS3) was expressed in 293E cells. The C-terminal includes human IgG1-Fc-Avi tag. |
|---|---|
| Molecular Mass | The 603 amino acid recombinant protein has a predicted molecular mass of approximately 66.7 kD. The DTT-reduced and non-reduced proteins migrates at approximately 70 - 90 kD and 140 - 200 kD by SDS-PAGE, respectively. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH7.2. |
| Endotoxin Level | Less than 0.1 EU per µg of protein as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Activity | Immobilized recombinant human Nectin-1 binds to Biotinylated Recombinant Human Nectin-3 in a dose-dependent manner. The ED50 for this effect is 1.4 – 8.4 ng/mL. |
|---|
| Application | Bioassay |
|---|
| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
|---|
| Structure | Dimer |
|---|
| Distribution | Epithelial cells, granule cells, APC, cancer cells, T-cells, and neuronal cells |
|---|
| Function | Adhesion, Ocular development involving the ciliary body |
|---|
| Interaction | T-lymphocytes, NK cell, epithelial cells |
|---|
| Ligand/Receptor | Nectin-1, -2, and -3, TIGIT, and CD155 |
|---|
| Bioactivity | Measured by its ability to bind recombinant human Nectin-1 |
|---|
| Cell Type | Antigen-presenting cells, Endothelial cells, Epithelial cells, Neurons, NK cells, T cells |
|---|
| Biology Area | Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity |
|---|
| Molecular Family | Adhesion Molecules, Innate Immune Signaling |
|---|
| Antigen References | Takai, Y. and H. Nakanishi. 2003. J Cell Sci. 116:17-27. Takai Y, et al. 2008. Annual Review of Cell and Developmental Biology. 24:309-42. De Agustín-Durán D, et al. 2021. Cells. 10:118. Satoh-Horikawa K, et al. 2000. J Biol Chem. 275:10291-9. Inagaki M, et al. 2005. Development. 132:1525-37. Inagaki M, et al. 2006. Genes Cells. 11:1125-32. |
|---|
| Gene ID | 25945 |
|---|
| UniProt | View information about Nectin3 on UniProt.org |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Nectin3, Nectin-3, PVRL3, PVRR3, CD113, CD113 antigen, nectin 3, poliovirus receptor-related 3, poliovirus receptor-related protein3, PPR3, PRR3CD113 |
|---|
