Biolegend, 568506, Biotinylated Recombinant Human B7-H6-Fc Chimera (carrier-free)
100microg
Catalog Number: 568506
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| Source | Biotinylated recombinant human B7-H6, amino acid (Asp25-Ser262) (Accession # Q68D85.1), with a linker (IEGRMD), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in CHO cells. Human B7-H6-Avi tag was site-specifically biotinylated by enzyme BirA. |
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| Molecular Mass | The 490 amino acid recombinant protein has a predicted molecular mass of approximately 55.14 kD. The DTT-reduced and non-reduced protein migrates at approximately 65-115 kD and 140-240 kD by SDS-PAGE. The predicted N-terminal amino acid is Asp. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, 5% glycerol and 2 mM EDTA |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | When recombinant human NKp30 is immobilized at 1 µg/mL, biotinylated recombinant human B7-H6-Fc Chimera binds in a dose-dependent manner with ED50 of 2.5 – 15 ng/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Dimer |
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| Distribution | Human tumor cell lines, cancer cells, monocytes and neutrophils after inflammatory stimulation, glioma stem-like cells. Not detected in healthy cells |
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| Function | Natural killer (NK) cell activation and cytotoxicity |
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| Interaction | NK cells |
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| Ligand/Receptor | NKp30 (CD337) |
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| Bioactivity | When recombinant human NKp30 is immobilized at 1 µg/mL, biotinylated recombinant human B7-H6-Fc Chimera binds in a dose-dependent manner with ED50 of 2.5 – 15 ng/mL. |
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| Cell Type | Leukemia, Monocytes, Neutrophils |
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| Biology Area | Cancer Biomarkers, Cell Death, Immuno-Oncology, Immunology |
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| Antigen References | Brandt CS, et al. 2009. J Exp Med. 206:1495-503. Kaifu T, et al. 2011. Cell Mol Life Sci. 68:3531-9. Matta J, et al. 2013. Blood. 122:394-404. Schlecker E, et al. 2014. Cancer Res. 74:3429-40. Chen Y, et al. 2018. Pathol Oncol Res. 24:717-721. Gutierrez-Franco J, et al. 2018. Immunobiology. 223:57-63. Obiedat A, et al. 2020. J Mol Med (Berl). 98:135-148. Chen H, et al. 2020. J Immunol Res. 2020:2328675. |
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| Gene ID | 374383 |
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| UniProt | View information about B7-H6 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | B7H6, NCR3LG1, natural killer cell cytotoxicity receptor 3 ligand 1, B7 Homolog 6 |
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