Biolegend, 570109, Recombinant Human TNF-α (ELISA Std.)

Name: Recombinant Human TNF-α (ELISA Std.)
Brand: Biolegend
SKU: 570109

4pack

Catalog Number: 570109

Pricing

Source	Human TNF-alpha, amino acids Val77-Leu233 (Accession # HUMTNFAB) was expressed in E. coli.
Molecular Mass	The 157 amino acid recombinant protein has a predicted molecular mass of 17,352 Da. The DTT-reduced and non-reduced protein migrates at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Val.
Purity	>98%, as determined by SDS-PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation	Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level	Less than 0.01ng per µg cytokine as determined by the LAL method.
Concentration	Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling	Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.

Ordering

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