Biolegend, 570509, Recombinant Human IL-17A (ELISA Std.)
4pack
Catalog Number: 570509
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| Source | Human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli and renatured from inclusion bodies. |
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| Molecular Mass | The 137 amino acid N-terminal methionylated protein has a predicted molecular mass of 15,666 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 16kDa by SDS-PAGE. The non-reduced protein migrates |
| Purity | Purity is >98% as determined by SDS PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain. |
| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
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| Application | ELISA |
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| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/ml to 15 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This IL-17A protein is useful as a standard in a human IL-17A sandwich ELISA in conjunciton with the monoclonal antibody pair: unlabeled purified clone BL127 antibody (capture) and biotinylated clone BL23 antibody (detection). |
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| Product Citations | Haug V, et al. 2020. Transplantation. e260:104. PubMed |
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| Structure | Cytokine; dimer |
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| Cell Sources | activated T cell subpopulation |
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| Cell Targets | Fibroblasts, epithelial and endothelial cells, stromal cells |
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| Receptors | IL-17R (CDw217) |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Hirota K, et al. 2007. J Exp Med. 204:41. 2. Furuzawa-Carballeda J, et al. 2007. Autoimmun Rev. 6:169. 3. Witowski J, et al. 2007. Kidney Int. 71:514. 4. Gaffen SL, et al. 2006. Vitam Horm. 74:255. 5. Hymowitz S, et al. 2001. EMBO J. 20:5332. |
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| Gene ID | 3605 |
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| UniProt | View information about IL-17A on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin 17A, IL17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8) |
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