Biolegend, 570814, Cell-Vive™ GMP Recombinant Human IL-6 (carrier-free)
25microg
Catalog Number: 570814
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| Source | Human IL-6, amino acids Pro29-Met212 (Accession # NM_000600) was expressed in E. coli. |
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| Molecular Mass | The 185 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21 kD. The DTT-reduced and non-reduced protein migrate at approximately 23 kD by SDS-PAGE. |
| N-terminalSequence Analysis | Pro-Val-Pro-Pro-Gly-Glu-Asp-Ser-Lys-Asp |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.1 µm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2. |
| Endotoxin Level | Less than or equal to 0.1 EU per µg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/µg by ELISA |
| Concentration | 500 µg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | ED50 = 4 - 20 pg/mL, as determined by a dose-dependent stimulation in a 7TD1 cell proliferation assay. The specific activity of Cell-Vive™ GMP Recombinant Human IL-6 (carrier-free) is >3.5 x 107 IU/mg when compared against the WHO International Standard for Human IL-6 (NIBSC code: 89/548). |
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| Application | Bioassay Cell cultures |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Distribution | IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells. |
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| Function | Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, and IL-17 and downregulated by glucocorticoids IL-4 and TGF-β |
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| Interaction | T cells, B cells, hepatocytes, and cholinergic neurons |
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| Ligand/Receptor | Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R |
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| Cell Type | Hematopoietic stem and progenitors |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | McClintock JY and Wagner EM. 2005. J. Appl. Physiol. 99:861. Murakami M, et al. 1993. Science 260:1808. Cartmell T, et al. 2000. J. Physiol. 526:653. Jones SA, et al. 2001. FASEB J. 15:43. Fielding CA, et al. 2008. J. Immunol. 181:2189. Sonderegger I, et al. 2008. Eur. J. Immunol. 38:1833. Erta M, et al. 2012. Int. J. Biol. Sci. 8:1254. Rosean TR, et al. 2014. Immunol. Res. 59:188. |
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| Gene ID | 3569 |
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| UniProt | View information about IL-6 on UniProt.org |
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| Other Names | Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF) |
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