Biolegend, 571214, Cell-Vive™ GMP Recombinant Human IL-21 (carrier-free)
25microg
Catalog Number: 571214
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| Source | Human IL-21, amino acid Gln30-Ser162 (Accession # NM_021803), was expressed in E.coli. |
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| Molecular Mass | The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Glutamine. |
| N-terminalSequence Analysis | Gln-Gly-Gln-Asp-Arg-His-Met-Ile-Arg-Met |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.1 µm filtered protein solution is in PBS, 1 mM EDTA, pH 7.2. |
| Endotoxin Level | Less than or equal to 0.1 EU per µg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/μg by ELISA |
| Concentration | 500 µg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Assay 1: ED50 = 0.6 - 3.0 ng/mL as measured by its ability to induce dose-dependent secretion of IFN-γ by NK-92 cells. ELISA MAX™ Deluxe Set Human IFN-γ (Cat. No. 430104) was used to measure IFN-γ secretion. Assay 2: GMP Cell-Vive™ recombinant human IL-21 induces the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.05 - 0.5 µg/mL. |
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| Application | Bioassay Cell Culture |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Interleukin |
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| Distribution | CD4 activated T cells, Th17 cells, NK T cells |
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| Function | IL-21 induces T- and B-cell proliferation, B-cell Ig class switching to IgG production, decreases dendritic cell function, enhances differentiation of effector and central memory T cells, and regulates T-cell and hematopoietic progenitor cell homeostasis. |
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| Interaction | T cells, B cells, NK cells, and dendritic cells as well as some nonimmune cells, such as fibroblasts and epithelial cells |
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| Ligand/Receptor | IL-21R and the common γc subunit (CD132) are components of the IL-21R complex. |
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| Bioactivity | Measured by its ability to induce IFN-γ secretion by NK-92 cells. |
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| Cell Type | B cells, Dendritic cells, Hematopoietic stem and progenitors, Tregs |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | Parrish-Novak J, et al. 2000. Nature. 408:57. De Totero D, et al. 2006. Blood. 107:3708. Korn T, et al. 2007. Nature. 448:484. Coquet JM, et al. 2008. J. Immunol. 180:7097. Dienz O, et al. 2009. J. Exp. Med. 206:69. Kaplan MH, et al. 2011. Blood. 117:6198. Eto D, et al. 2011. PLoS One. 6:e17739. |
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| Gene ID | 59067 |
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| UniProt | View information about IL-21 on UniProt.org |
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| Other Names | Interleukin 21, Interleukin-21, IL21 |
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