Biolegend, 571309, Recombinant Human IL-22 (ELISA Std.)
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Catalog Number: 571309
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| Source | Human IL-22, amino acids Ala34-Ile179 (Accession# NM_020525) was expressed in E. coli. |
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| Molecular Mass | The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16.88 kD. The DTT-reduced protein migrates at approximately 15 kD and the non-reduced protein migrates at approximately 14 kD by SDS-PAGE. |
| Purity | Purity is >98%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, and protease inhibitors. |
| Endotoxin Level | Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
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| Application | ELISA - Quality tested |
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| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 8000 pg/ml to 125 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This IL-22 protein is useful as a standard in a human IL-22 sandwich ELISA, using unlabeled poly5161 antibody (Cat. No. 516101) for capture and biotinylated poly5161 antibody (Cat. No. 516103) for detection. |
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| Structure | Cytokine |
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| Distribution | Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells |
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| Function | Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis |
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| Interaction | Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma |
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| Ligand/Receptor | IL-22R1 and IL-10R2 receptor chains |
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| Bioactivity | IL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cells |
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| Biology Area | Stem Cells, Immunology, Cell Biology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Nagalakshmi ML, et al. 2004. Intern. Immunopharmacol. 4:679. 2. Kebir H, et al. 2007. Nat. Med. 13:1173. 3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807. 4. Pene J, et al. 2008. J. Immunol. 180:7423. 5. Dumoutier L, et al. 2000. J. Immunol. 164:1814. 6. Xie MH, et al. 2000. J. Biol. Chem. 265:31335. 7. Kotenko SV, et al. 2001. J. Immunol. 166:7096. 8. Chung Y, et al. 2006. Cell Res. 16:902. 9. Trifari S, et al. 2009. Nat. Immunol. 10:864. |
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| Gene ID | 50616 |
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| UniProt | View information about IL-22 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18 |
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