Biolegend, 571809, Recombinant Human IL-4 (ELISA Std.)
4pack
Catalog Number: 571809
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| Source | E. coli, approximately 15 kD |
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| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Endotoxin Level | Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
| Application | ELISA |
| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This IL-4 protein is useful as a standard for a human IL-4 sandwich ELISA, using unlabeled 8D4-8 antibody (catalog #500702) for capture and biotinylated MP4-25D2 antibody (catalog #500804) for detection. |
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| Product Citations | Serre K, et al. 2011. PLoS One. 6:e20731. PubMed |
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| Structure | Cytokine, 14.9 kD |
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| Bioactivity | Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells |
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| Cell Sources | Mast cells, T cells, bone marrow stromal cells |
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| Cell Targets | B cells, T cells, monocytes, endothelial cells, fibroblasts |
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| Receptors | Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R |
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| Biology Area | Stem Cells, Immunology, Cell Biology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens H, et al. 1991. In vivo 5:567. 4. Paul W. 1991. Blood 77:1859. |
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| Regulation | Upregulated by IL-2, platelet activating factor; downregulated by TGF-β |
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| Gene ID | 3565 |
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| UniProt | View information about IL-4 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2) |
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