Biolegend, 574814, Cell-Vive™ GMP Recombinant Human M-CSF (carrier-free)
25microg
Catalog Number: 574814
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| Source | Human M-CSF, amino acids Glu33-Ser190 (Accession# NM_172212.2) was expressed in 293E cells. |
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| Molecular Mass | The 179 amino acid recombinant protein has a predicted molecular mass of approximately 20.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 25 - 35 kD and 55 - 70 kD respectively by SDS-PAGE. The N-terminal contains a His9-(SGGG)2-IEGR-tag. |
| N-terminalSequence Analysis | Glu-Glu-Val-Ser-Glu-Tyr-(Cys)-Ser-His-Met |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.1 µm filtered protein solution is in PBS. |
| Endotoxin Level | Less than or equal to 0.1 EU per μg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/μg by ELISA |
| Concentration | 500 µg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | ED50 = 0.5 - 2.0 ng/mL as measured by its ability to induce proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The specific activity of Cell-Vive™ GMP Recombinant Human M-CSF (carrier-free) is ≥ 9.4 x 107 IU/mg when compared against the WHO International Standard for human M-CSF (NIBSC code: 89/512). |
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| Application | Bioassay Cell cultures |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Disulfide-linked homodimer |
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| Distribution | Hematopoietic stem and progenitors, embryonic stem cells |
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| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. |
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| Interaction | Monocytes, macrophages, mononuclear phagocyte precursors, microglia, proliferating smooth muscle cells, umbilical vein endothelial cells, and breast cancer cell lines |
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| Ligand/Receptor | M-CSFR or CSF1R (CD115) |
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| Bioactivity | Measured by its ability to induce proliferation of M-NFS-60 cells. |
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| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors |
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| Biology Area | Cell Biology, Cell Proliferation and Viability, Immunology, Stem Cells |
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| Molecular Family | Cytokine/Chemokine Receptors, Growth Factors |
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| Antigen References | Kawasaki ES, et al. 1985. Science 230:291. Wei S, et al. 2010. J. Leukoc. Biol. 88:495. Hodge JM, et al. 2011. PloS One 6:e21462. Morandi A, et al. 2011. PloS One 6:e27450. Erblich B, et al. 2011. PloS One 6:e26317. MacDonald KP, et al. 2010. Blood 116:3955. |
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| Gene ID | 1435 |
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| UniProt | View information about M-CSF on UniProt.org |
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| Other Names | Colony Stimulating Factor 1, Colony Stimulating Factor 1 (Macrophage), Macrophage Colony Stimulating Factor 1, CSF-1, MCSF, CSF1 |
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