Biolegend, 575109, Recombinant Mouse IL-1β (ELISA Std.)
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Catalog Number: 575109
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| Source | Mouse IL-1β, amino acids Val118-Ser269 (Accession # NM_008361) was expressed in E. coli, approximately 17 kD. |
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| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
| Application | ELISA |
| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/ml to 31.3 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This IL-1ß protein is useful as a standard for a mouse IL-1ß sandwich ELISA, using unlabeled B122 antibody (Cat. No. 503502) for capture and biotinylated Poly5158 antibody (Cat. No. 515801) for detection. |
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| Product Citations | Vázquez-Zapién GJ, et al. 2022. Cells. 11: . PubMed Rissiek B, et al. 2017. Sci Rep. . 10.1038/s41598-017-16613-w. PubMed Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed |
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| Structure | Cytokine; 17.5 kD (Mammalian) |
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| Bioactivity | Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages |
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| Cell Sources | Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, |
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| Cell Targets | B cells, T cells, monocytes |
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| Receptors | Type I IL-1R (CDw121a), Type II IL-1R (CDw121b) |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095. |
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| Regulation | Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin |
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| Gene ID | 16176 |
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| UniProt | View information about IL-1beta on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF) |
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