Biolegend, 575709, Recombinant Mouse IL-6 (ELISA Std.)
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Catalog Number: 575709
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| Source | Mouse IL-6, amino acids Phe25-Thr211 (Accession# MUSIL6), was expressed in E. coli. |
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| Molecular Mass | The 188 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,866 Da. The DTT-reduced protein migrates at approximately 22kDa and the non-reduced protein migrates at approximately 21.5kDa by SDS-PAGE. |
| Purity | Purity is >98% as determined by SDS PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain. |
| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
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| Application | ELISA |
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| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This IL-6 protein is useful as a standard for a mouse IL-6 sandwich ELISA, using unlabeled MP5-20F3 antibody (Cat. No. 504502) for capture and biotinylated MP5-32C11 antibody (Cat. No. 504602) for detection. |
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| Product Citations | Li H, et al. 2019. Investig Clin Urol. 60:359. PubMed |
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| Structure | Cytokine |
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| Distribution | IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells. |
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| Function | Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β |
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| Interaction | T cells, B cells, hepatocytes, cholinergic neurons |
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| Ligand/Receptor | Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R |
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| Bioactivity | Hematopoiesis, antigen-specific immune responses, inflammatory reactions, acute phase reactions |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. McClintock JE and Wagner EM J Appl Physiol 99:861-866 2005. 2. Murakami M Science 260:1808-1810 1993. 3. Jones SA, et al. FASEB J. 15:43-58 2001. 4. Fielding CA, et al. J. Immunol. 181:2189-2195. 2008. 5. Sonderegger I, et al. Eur. J. Immunol. 38:1833-1838 2008. |
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| Gene ID | 16193 |
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| UniProt | View information about IL-6 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF) |
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