Biolegend, 575809, Recombinant Mouse IL-10 (ELISA Std.)
4pack
Catalog Number: 575809
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| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
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| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
| Application | ELISA |
| Recommended Usage | ELISA with capture antibody clone JES5-2A5 (Cat. No. 504901) or clone MK10A6 (Cat. No. 521801) and detection antibody clone JES5-16E3 (Cat. No. 505003), with an approximate standard range from 30 pg/mL to 4 ng/mL. |
| Product Citations | Vázquez-Zapién GJ, et al. 2022. Cells. 11: . PubMed |
| Structure | Acid-labile cytokine; dimer |
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| Bioactivity |
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| Inhibits IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppresses delayed type hypersensitivity response; stimulates TH2 cell response; mast cell proliferation i |
| Cell Sources | Activated CD8+ T cells, TH0, TH2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes |
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| Cell Targets | T cells, B cells, mast cells, macrophages |
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| Receptors | IL-10R (CDw210) |
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| Biology Area | Immunology, Cell Biology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314. 3. Howard M, et al. 1992. Immunol. Today. 13:198. 4. Quesniaux V. 1992. Research Immunol. 143:385. |
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| Regulation | Downregulated by IL-4, IL-10 |
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| Gene ID | 16153 |
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| UniProt | View information about IL-10 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF) |
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