Biolegend, 576209, Recombinant Mouse IL-22 (ELISA Std.)
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Catalog Number: 576209
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| Source | Mouse IL-22, amino acids Leu34-Val179 (Accessio # NM_016971) was expressed in E. coli. |
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| Molecular Mass | The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.8 kD. The DTT-reduced protein migrates at approximately 15 kD and the non-reduced protein migrates at approximately 13 kD by SDS-PAGE. |
| Purity | Purity is >98%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Endotoxin Level | Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
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| Application | ELISA - Quality tested |
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| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 3.9 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This IL-22 protein is useful as a standard in a mouse IL-22 sandwich ELISA, using unlabeled poly5164 antibody (Cat. No. 516401) for capture and biotinylated poly5164 antibody (Cat. No. 516407) for detection. |
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| Biology Area | Cell Biology, Immunology, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Dumoutier L, et al. 2000. J. Immun. 164:1814. 2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335. 3. Jones BC, et al. 2008. Structure. 4. Kotenko SV, et al. 2001. J. Immun. 166:7096. 5. Chang C, et al. 2006. Cell Research 16:902. |
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| Gene ID | 50929 |
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| UniProt | View information about IL-22 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-22, IL-TIF |
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