Biolegend, 576509, Recombinant Mouse MCP-1 (ELISA Std.)
4pack
Catalog Number: 576509
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Category
Pack Size105 units / pack
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| Source | Mouse MCP1, amino acids Gln24-Arg96 (Accession # NM_011333) was expressed in E. coli, approximately 13.8 kD |
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| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
| Application | ELISA |
| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1,000 pg/ml to 7.8 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This MCP-1 protein is useful as a standard for a mouse MCP-1 sandwich ELISA, using unlabeled 4E2/MCP antibody (Cat. No. 506004 ) for capture and biotinylated 2H5 antibody (Cat. No. 505908) for detection. |
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| Application References(PubMed link indicates BioLegend citation) | Liu Z, et al. 2013. J. Immunol. 191:857. PubMed |
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| Product Citations | Liu Z, et al. 2013. J Immunol. 191:857. PubMed |
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| Structure | Chemokine |
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| Distribution | MCP-1 (Ccl2) is secreted by fibroblast, endothelial cells, smooth muscle cells, tumor cells, phytohemaglutinin stimulated mononuclear cells, TNF-a stimulated osteoblastic MC3T3-E1 cells. |
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| Function | MCP-1 induces migration of monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells. MCP-1 is induced by IL-1b, TNF, IFNg, PDGF, IL-4, MIF, and IL-17. |
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| Ligand/Receptor | MCP-1 mediates its cellular effects primarily through CCR2 receptor |
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| Bioactivity | ED50 = 8 -15 ng/ml, corresponding to a specific activity of 1.25 — 0.6 x 105 units/mg, as determined by the dose dependent chemoattraction of THP-1 cells |
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| Biology Area | Cell Biology, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Loberg RD, et al. 2007 Cancer Research 67:9417-9424. 2. Gregory JL, et al. 2006 J Immunol 177:8072-8079. 3. Qui Z, et al. 2009 Immunology 214:835-842. 4. Reichel CA, et al. 2009 Arterioscler Thromb Vasc Biol. ATVBAHA.109.193268v1 5. McQuibban GA, et al. 2002 Blood 100:1160-1167 |
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| Gene ID | 20296 |
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| UniProt | View information about CCL2 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Monocyte chemotactic protein 1, chemokine (C-C motif) ligand 2, Ccl2, JE, Scya2, monocyte chemotactic and activating factor, MCAF. |
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