Biolegend, 577009, Recombinant Mouse IL-12 (p70) (ELISA Std.)
4pack
Catalog Number: 577009
Send an RFQ.
Category
Pack Size107 units / pack
Loading…
| Source | CHO cells, approximately 70 kD |
|---|---|
| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
| Application | ELISA |
| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| This IL-12 p70 protein is useful as a standard for a mouse IL-12 sandwich ELISA, using unlabeled C18.2 antibody (Cat.No. 511802 ) for capture and biotinylated C17.8 antibody (Cat. No. 505302 ) for detection. |
| Structure | Cytokine; heterodimer |
|---|
| Bioactivity | The ED50 was determined by the stimulation of IFN-γ production by murine splenocytes co-stimulated with IL-18 is ≤0.1 ng/ml, corresponding to a specific activity of ≥1 x 107 units/mg. |
|---|
| Cell Sources | Dendritic cells, B cells, T cells |
|---|
| Cell Targets | T cells, NK cells, PBL |
|---|
| Biology Area | Immunology, Innate Immunity |
|---|
| Molecular Family | Cytokines/Chemokines |
|---|
| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Quesniaux V. 1992. Research Immun. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res.4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. |
|---|
| Regulation | Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23 |
|---|
| Gene ID | 1615916160 |
|---|
| UniProt | View information about IL-12 p70 on UniProt.org |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | NKSF, CTL maturation factor (TCMF), cytotoxic lymphocyte maturation factor (CLMF), TSF |
|---|
