Biolegend, 578616, Cell-Vive™ GMP Recombinant Human G-CSF (carrier-free)

Name: Cell-Vive™ GMP Recombinant Human G-CSF (carrier-free)
Brand: Biolegend
SKU: 578616

100microg

Catalog Number: 578616

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Category

Amino Acids

Description

Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

Specifications

General

Source	Human G-CSF, amino acids Thr27-Pro200 (Accession# BC033245.1) was expressed in 293E cells.
Molecular Mass	The 174 amino acid recombinant protein has a predicted molecular mass of 18.6 kD. The DTT-reduced and the non-reduced proteins migrate at approximately 20 kD by SDS-PAGE. The N-terminal amino acid is Thr.
N-terminalSequence Analysis	Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro
Purity	≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation	0.1 µm filtered protein solution is in PBS.
Endotoxin Level	Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content	≤0.500 ng/μg by ELISA
Concentration	500 µg/mL
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

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Biolegend, 578616, Cell-Vive™ GMP Recombinant Human G-CSF (carrier-free)

Send an RFQ.

General

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