Biolegend, 579414, Cell-Vive™ GMP Recombinant Human IL-1β (carrier-free)
25microg
Catalog Number: 579414
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| Source | Human IL-1β, amino acids Ala117-Ser269 (Accession # NM_000576) was expressed in E. coli. |
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| Molecular Mass | The 153 amino acid recombinant protein has a predicted molecular mass of 17.4 kD. The DTT-reduced and the non-reduced protein migrate at approximately 18 kD by SDS-PAGE. The N-terminal amino acid is Alanine. |
| N-terminalSequence Analysis | Ala-Pro-Val-Arg-Ser-Leu-Asn-(Cys)-Thr-Leu |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.1 µm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2. |
| Endotoxin Level | Less than or equal to 0.1 EU per µg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/µg by ELISA |
| Concentration | 500 µg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | ED50 = 2.5 - 12.5 pg/mL, as determined by a dose-dependent stimulation in a proliferation assay with D10.G4.1 mouse helper lymphocytes. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The specific activity of Cell-Vive™ GMP Recombinant Human IL-1β (carrier-free) is ≥ 4.5 x 107 IU/mg when compared against the WHO International Standard for Human IL-1β (NIBSC code: 86/680). |
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| Application | Bioassay Cell Culture |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Distribution | IL-1β is expressed in activated monocytes/macrophages. |
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| Function | Interleukin (IL)-1β is a cytokine with a key role in the pathophysiology of local and systemic inflammation. IL-1β induces cytokine, chemokine, proinflammatory molecule secretion, and adhesion molecule expression in diverse cells. |
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| Interaction | Hematopoietic stem and progenitors |
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| Ligand/Receptor | Type I and II IL-1 receptors (IL-1RI and IL-1RII) |
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| Bioactivity | Measured by its ability to induce proliferation of D10.G4.1 mouse helper lymphocytes |
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| Cell Type | Hematopoietic stem and progenitors |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | Stevenson FT, et al. 1992. J Cell Physiol 152:223-231. Shi J, et al. 2007. J. Immunol. 179:1245-1253. Dinarello CA. 2006. Cancer Metastasis Rev 25:307-313. Boost KM, et al. 2008. BMC Cancer 8:265. |
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| Gene ID | 3553 |
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| UniProt | View information about IL-1beta on UniProt.org |
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| Other Names | Catabolin, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta. |
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