Biolegend, 581809, Recombinant Human IL-33 (ELISA Std.)
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Catalog Number: 581809
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Pack Size107 units / pack
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| Source | Human IL-33, amino acids Ser112-Thr270 (Accession# NM_ 033439) was expressed in E. coli. |
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| Molecular Mass | The 159 amino acid recombinant protein has a predicted molecular mass of approximately 18 kD. The DTT-reduced protein migrates at approximately 19 kD and non-reduced protein migrates at approximately 20 kD by SDS-PAGE. The N-terminal amino acid is Serine. |
| Purity | >98%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
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| Application | ELISA - Quality tested |
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| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1000 pg/ml to 15.6 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | This IL-33 protein is useful as a standard in a human IL-33 sandwich ELISA, using unlabeled poly5163 antibody (Cat. No. 516301) for capture and biotinylated poly5163 antibody (Cat. No. 516303) for detection. |
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| Distribution | IL-33 mRNA is expressed in a broad range of human tissues, such as heart, small intestine, brain, ovary, testis, pancreas, and colon. Higher IL-33 expression was detected in human keratinocyte, fibroblast, myocyte, and alveolar cells. |
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| Function | IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-α and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes. |
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| Ligand/Receptor | IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein). |
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| Bioactivity | Activity was tested by D10.G4.1 cell proliferation induced by human IL-33. The ED50 is 0.05 to 0.2 ng/ml, corresponding to a specific activity of 5 x 106 to 2 x 107 units/mg. |
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| Biology Area | Cell Biology, Immunology, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Schmitz J, et al. 2005. Immunity 23:479. 2. Barksby HE, et al. 2007. Clin. Exp. Immunol. 149:217. 3. Arend WP, et al. 2008. Immunol. Rev. 223:20. 4. Suzukawa M, et al. 2008. J. Immunol. 181:5981. 5. Moussion C, et al. 2008. PLoS One 3:e3331. 6. Mildner M, et al. 2010. Cardiovasc. Res. 87:769. |
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| Gene ID | 90865 |
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| UniProt | View information about IL-33 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | IL-1F11 |
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