Biolegend, 581914, Cell-Vive™ GMP Recombinant Human IL-7 (carrier-free)
25microg
Catalog Number: 581914
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| Source | Human IL-7, amino acids Asp26-His177 (Accession # NP_000871.1) was expressed in 293E cells. |
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| Molecular Mass | The 165 amino acid recombinant protein has a predicted molecular mass of approximately 18.8 kD. The DTT-reduced and non-reduced protein migrate at approximately 20 - 30 kD by SDS-PAGE. The C-terminal contains an 8His-(TG8HGGQ)-tag. |
| N-terminalSequence Analysis | Asp-(Cys)-Asp-Ile-Glu-Gly-Lys-Asp-Gly-Lys |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.1 µm filtered protein solution is in PBS, pH 7.2. |
| Endotoxin Level | Less than or equal to 0.1 EU per μg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/µg by ELISA |
| Concentration | 500 µg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | ED50 = 0.2 – 1.2 ng/mL, as determined by a dose-dependent stimulation in a proliferation assay with PHA-activated human peripheral blood lymphocytes (PBL). Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The specific activity of Cell-Vive™ GMP Recombinant Human IL-7 (carrier-free) is >1 x 108 IU/mg when compared against the WHO International Standard for Human IL-7 (NIBSC code: 90/530). |
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| Application | Bioassay Cell Culture |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Distribution | IL-7 is produced by epithelial cells in thymus, bone marrow, and intestine. Additional sites of IL-7 production include epithelial goblet cells, keratinocytes, fetal liver, adult liver, dendritic cells, skeletal muscle cells, fibroblastic reticular cells, and follicular dendritic cells. |
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| Function | IL-7 induces proliferation of human immature B cells, and it is critical for T-cell development and peripheral T-cell homeostasis. Regulates T folicular helper cells. |
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| Interaction | B cells, Hematopoietic stem and progenitors |
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| Ligand/Receptor | IL-7Rα (CD127), common gamma chain (γc or CD132) |
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| Bioactivity | Measured by its ability to induce proliferation of 7TD1 cell |
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| Cell Type | B cells, Dendritic cells, Epithelial cells, Leukemia, Lymphocytes, NK cells, T cells, Tfh, Thymocytes |
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| Biology Area | Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Stem Cells |
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| Molecular Family | Cytokine/Chemokine Receptors, Cytokines/Chemokines |
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| Antigen References | Park LS, et al. 1990. J. Exp. Med. 171:1073. Fry TJ and Mackall CL. 2002. Blood 99:3892. Lai L, et al. 2006. Blood 107:1776. Link A, et al. 2007. Nat. Immunol. 8:1255. Wofford J, et al. 2008. Blood 111:2101. Parrish YK, et al. 2009. J. Immunol. 182:4255. Saini M, et al. 2009. Blood 113:5793. Guimond M, et al. 2009. Nat. Immunol. 10:149. |
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| Gene ID | 3574 |
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| UniProt | View information about IL-7 on UniProt.org |
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| Other Names | Interleukin 7, Lymphopoietin-1 (LP-1), IL7 |
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