Biolegend, 582414, Cell-Vive™ GMP Recombinant Human TSLP (carrier-free)
50microg
Catalog Number: 582414
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| Source | Human TSLP, amino acids Met-Tyr29-Gln159 (Accession# AF338732), was expressed in E. coli. |
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| Molecular Mass | The 132 amino acid recombinant protein has a predicted molecular mass of approximately 15 kD. The DTT-reduced protein migrates at approximately 17 kD and non-reduced protein at approximately 18 kD by SDS-PAGE. The N-terminal amino acid is Met. |
| N-terminalSequence Analysis | Met-Tyr-Asp-Phe-Thr-Asn-(Cys)-Asp-Phe-Glu |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | Protein was lyophilized from 0.1 μm filtered solution containing 20 mM sodium phosphate, 150 mM NaCl, pH 7.2 |
| Endotoxin Level | Less than or equal to 0.1 EU per μg of protein as determined by LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/μg by ELISA |
| Concentration | 50 µg and 500 µg sizes are lyophilized |
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| Storage & Handling |
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| Unopened lyophilized vials can be stored at ≤ -20°C for 5 years and between 2°C – 8°C for 2 weeks. Reconstituted aliquots can be stored between -20°C to -70°C for 3 months and between 2°C – 8°C for 2 weeks. Reconstitute lyophilized protein in sterile water. Before reconstitution, make sure sterile water and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer to the vial, to obtain the recommended stock concentration of 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. |
| Activity | Cell-Vive™ GMP Recombinant Human TSLP induces proliferation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cells in a dose dependent manner. The ED50 for this effect is 0.4 – 2.4 ng/mL. |
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| Application | Bioassay |
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| Application Notes | Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Cytokine |
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| Ligand/Receptor | Heterodimeric receptor complex composed of the IL-7 receptor α chain (IL-7Rα) and the TSLP receptor (TSLPR or CRLF2) |
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| Bioactivity | Induces proliferation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cells in a dose dependent manner |
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| Cell Sources | Epithelial cells, mast cells, basophils, epidermal keratinocytes in atopic dermatitis, synovial fibroblast in arthritis rheumatoid, human airway smooth muscle cells |
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| Cell Targets | Monocyte, dendritic cells, activated human basophils, activated human T cells, Langerhans cells, B cells, mast cells, activated pulmonary Tregs, NKT cells |
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| Antigen References | Levin SD, et al. 1999. J. Immunol. 162:677. Reche PA, et al. 2001. J. Immunol. 167:336. Omori M and Steven Ziegler. 2007. J. Immunol. 178:1396. Rochman I, et al. 2007. J. Immunol. 178:6720. Mazzuchelli R, et al. 2008. Blood. 112:3283. Siracusa MC, et al. 2011. Nature. 477:229. Nguyen KD, et al. 2010. Allergy Asthma Clin. Immunol. 6:4. |
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| Regulation | Induces proliferation of naïve T cells, the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes, and the T cell stimulatory capacity of CD11c+ subset of dendritic cells; drives Th2 differentiation, drives Th2 cytokine-mediated inflammation to promote basophil hematopoiesis |
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| Gene ID | 85480 |
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| UniProt | View information about TSLP on UniProt.org |
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| Other Names | Thymic stromal lymphopoietin (TSLP) |
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