Biolegend, 591909, Recombinant Human CTLA-4 (ELISA Std.)
4pack
Catalog Number: 591909
Send an RFQ.
Category
Loading…
| Source | Accession# Q6GR94 |
|---|---|
| Molecular Mass | 40 kD (monomer) |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Endotoxin Level | <1.0 EU per 1 µg of the protein by the LAL method. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
| Application | ELISA - Quality tested |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Recommended Usage |
|---|
| Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of two-fold dilutions from 2500 pg/mL to 39 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | This CTLA-4 protein is useful as a standard for a human CTLA-4 sandwich ELISA, using unlabeled A3.6B10.G1 antibody (Cat. No. 525401) for capture and biotinylated L3D10 antibody (Cat. No. 349911) for detection. |
|---|
| Structure | Disulfide-linked homodimer |
|---|
| Distribution | Activated T cells and B cells |
|---|
| Function | Negative regulator of T cell activation |
|---|
| Ligand/Receptor | B7-1 (CD80), B7-2 (CD86) |
|---|
| Cell Type | B cells, Dendritic cells, Tregs |
|---|
| Biology Area | Immunology, Inhibitory Molecules |
|---|
| Molecular Family | Soluble Receptors, CD Molecules, Immune Checkpoint Receptors |
|---|
| Antigen References | 1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego. 2. Kuiper H, et al. 1995. J. Immunol. 155:1776. 3. Lindsten T, et al. 1993. J. Immunol. 151:3489. 4. Morton P, et al. 1996. J. Immunol. 156:1047. |
|---|
| Gene ID | 1493 |
|---|
| UniProt | View information about CTLA-4 on UniProt.org |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Cytotoxic T-Lymphocyte Antigen 4 (CTLA4) |
|---|
