Biolegend, 592114, Cell-Vive™ GMP Recombinant Human IL-18 (carrier-free)

Name: Cell-Vive™ GMP Recombinant Human IL-18 (carrier-free)
Brand: Biolegend
SKU: 592114

25microg

Catalog Number: 592114

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Category

Amino Acids

Description

IL-18 was first identified as IFN-β-inducing factor (IGIF). It is a member of the IL-1 family of cytokines and similar to IL-1β, IL-18 is initially synthesized as an inactive precursor without a signal peptide and is cleaved into the mature form by activated caspase 1. Caspase I-independent maturation of IL-18 is induced by FASL. Mature IL-18 binds directly to the IL-18 receptor alpha chain and then recruits IL-18 receptor beta chain to form a high affinity complex. The high affinity complex recruits MyD88 and leads to IRAK/TRAF6 pathway activation and NF-κB nuclear translocation. IL-18 in combination with IL-12 shows a synergistic effect on IFNβ production. Without costimulation, IL-18 alone does not induce IFNβ production. IL-18 can induce IFNβ production from splenocytes, liver macrophages, T lymphocytes, and natural killer cells. IL-18 also enhances the production of GM-CSF and IL-12. IL-18 enhances Th1 cell development by synergizing with IL-12 and promotes Th2 cell differentiation in the presence of TCR activation. IL-18 plays a major role in autoimmune and inflammatory diseases. It has been implicated in many diseases such as eczema, psoriasis, inflammatory bowel disease, metabolic syndromes, hemophagocytic syndrome, sepsis, and acute kidney injury. Blocking of IL-18 activity has been an attractive therapeutic approach for autoimmune disease. It has been shown that neutralization of IL-18 has reduced both intestinal IFNβ and TNFα production and resulted in a dose dependent reduction in colitis severity in mice. IL-18 is also able to induce angiogenesis, migration, proliferation and immune escape, and has been associated with cancer. Several publications show that IL-18 gene polymorphism may be risk factors for several cancers. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

Specifications

General

Source	Human IL-18, amino acids Tyr 37-Asp193 (Accession# NP-001553) was expressed in E. coli.
Molecular Mass	The 157 amino acid recombinant protein has a predicted molecular mass of approximately 18 kD. The DTT-reduced and non-reduced protein migrates at approximately 18 kD by SDS-PAGE. The predicted N-terminal amino acid is Tyr.
N-terminalSequence Analysis	Tyr-Phe-Gly-Lys-Leu-Glu-Ser-Lys-Leu-Ser
Purity	≥ 95%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.1 μm filtered protein solution is in PBS pH 7.2 with 10mM DTT, 1mM EDTA, and 10% Glycerol
Endotoxin Level	Less than or equal to 0.1 EU per μg of protein as determined by the LAL method.
Residual Host Cell Protein Content	≤ 0.500 ng/μg by ELISA
Concentration	25 μg & 100 μg sizes are bottled at 0.5 mg/mL
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles

Ordering

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Biolegend, 592114, Cell-Vive™ GMP Recombinant Human IL-18 (carrier-free)

Send an RFQ.

General

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