Biolegend, 592116, Cell-Vive™ GMP Recombinant Human IL-18 (carrier-free)
100microg
Catalog Number: 592116
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| Source | Human IL-18, amino acids Tyr 37-Asp193 (Accession# NP-001553) was expressed in E. coli. |
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| Molecular Mass | The 157 amino acid recombinant protein has a predicted molecular mass of approximately 18 kD. The DTT-reduced and non-reduced protein migrates at approximately 18 kD by SDS-PAGE. The predicted N-terminal amino acid is Tyr. |
| N-terminalSequence Analysis | Tyr-Phe-Gly-Lys-Leu-Glu-Ser-Lys-Leu-Ser |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.1 μm filtered protein solution is in PBS pH 7.2 with 10mM DTT, 1mM EDTA, and 10% Glycerol |
| Endotoxin Level | Less than or equal to 0.1 EU per μg of protein as determined by the LAL method. |
| Residual Host Cell Protein Content | ≤ 0.500 ng/μg by ELISA |
| Concentration | 25 μg & 100 μg sizes are bottled at 0.5 mg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles |
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| Activity | The ED50 is 1.5 - 9 ng/mL, as determined by a dose-dependent induction of IFNγ secretion by KG-1 cells. The specific activity is ≥2.3 x 106 IU/mg when compared against the WHO International Standard for Human IL-18 (NIBSC code: 03/200). |
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| Application | Bioassay Cell Culture |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Application References(PubMed link indicates BioLegend citation) | Orzalli MH, et al. 2018. Mol Cell. 71:825. PubMed Foltz JA, et al. 2018. Cancers (Basel). 10:. PubMed Stebbing J, et al. 2021. Sci Adv. 7:00. PubMed |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Cytokine |
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| Distribution | The major sources of IL-18 are macrophages and dendritic cells. IL-18 precursor is constitutively expressed in endothelial cells, keratinocytes, and intestinal epithelial cells. It is also expressed by renal parenchymal cells (tubular epithelial cells, podocytes, and mesangial cells). |
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| Function | IL-18 is essential for both innate and adaptive immunity. IL-18 induces synthesis of TNF, IL-1, Fas ligand, and several inflammatory chemokines. VEGF-D increases the expression and the secretion of IL-18 from gastric cancer cell lines. IL-18 binding protein (IL-18BP) binds to mature IL-18 with high affinity and prevents its interaction with IL-18Ra. IL-1F7 is a negative regulator of IL-18. IL-18 mRNA is induced by stress and bacteria in adrenal cortex and mouse osteoblasts respectively. |
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| Interaction | CD4 and CD8 lymphocytes, NK cells, splenocytes, and smooth muscle cells. |
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| Ligand/Receptor | IL-18 receptor (heteromeric complex of alpha and beta subunits). |
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| Bioactivity | IL-18 induces IFNγ secretion by KG-1 cells. |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | Okamura H, et al. 1995. Nature. 378:88-91. Siegmund B, et al. 2001. Am J Physiol Regul Integr Comp Physiol. 281:R1264-73. Rodriguez-Galán MC, et al. 2005. J Immunol. 174:2796-804. Alboni S, et al. 2010. J Neuroinflammation. 7:9. Dinarello CA, et al. 2013. Front Immunol. 4:289. Fabbi M, et al. 2015. J Leukoc Biol. 97:665-75. |
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| Gene ID | 3606 |
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| UniProt | View information about IL-18 on UniProt.org |
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| Other Names | Interferon-gamma-inducing factor, IGIF, IL-1g, IL18, IL1F4 |
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