Biolegend, 598512, AZDye 647 Recombinant Human CXCL12
10microg
Catalog Number: 598512
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| Source | Human CXCL12, amino acids Lys22-Lys89 (Accession # P48061), with a C-terminal AZDye647, was expressed in E. coli. |
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| Molecular Mass | The 93 amino acid recombinant protein has a predicted molecular weight mass of 9.9 kDa. |
| Purity | > 95%, as determined by mass spectrometry |
| Formulation | Lyophilized from 0.2 µm filtered solution of protein in DI-water. |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | Lyophilized, 10 µg per vial |
| Storage & Handling | Lyophilized at -20°C to -80°C until expirationLyophilized at 2°C to 8°C for 2 weeksReconstituted at 2°C to 8°C for 2 weeksReconstituted at -20°C to -80°C for 3 monthsReconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared at concentrations of 250 µg/mL up to 1 mg/mL in 0.22 µm filtered DI-water. Avoid repeated freeze/thaw cycles. |
| Activity | Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CXCR4. The ED50 for this effect was measured at 4 to 10 ng/mL. |
| Application | FC, Live cell imaging |
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| Application Notes |
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| Protein has been verified for bioactivity prior to conjugation |
| Structure | CXCL12 is a 93 amino acids molecule with a predicted molecular weight of 9.9 kDa. PDB ID – 2J7Z |
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| Distribution | CXCL12 is expressed by intestinal epithelial cells, human brain micro vessel endothelial cells (HBMEC), bone marrow stromal and epithelial cells |
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| Function | CXCL12 is chemotactic for both peripheral blood T cells and lamina propia T cells. CXCL12 is chemotactic for peripheral blood B cells from healthy and RA donors. CXCL12 and its receptor are involved in the mobilization of CD34+ progenitors to the peripheral blood and the homing of stem cells to their specific niches. CXCL12 induces proliferation in pancreatic intraepithelial neoplasia cells (PanIN). CXCL12 is a constitutive chemokine involved in lung, brain, joint, brain, and intestine inflammation |
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| Interaction | SDF-1 binds to the CXCR4-expressed cells such as T lymphocytes, B cells monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, and vascular smooth muscle cells (VSMCs) |
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| Ligand/Receptor | CXCL12 binds to CXCR4 receptor. |
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| Bioactivity | Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CXCR4. The ED50 for this effect was measured at 4 to 10 ng/mL |
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| Cell Type | Leukocytes, Lymphocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, Platelets |
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| Biology Area | Cell Biology, Immuno-Oncology, Immunology, Innate Immunity |
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| Molecular Family | Cytokines/Chemokines, Innate Immune Signaling |
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| Antigen References | Shirozu M, et al. 1995. Genomics. 28:495-500. Dar A, et al. 2006. Exp Hemathol. 34:967-75. Müller A, et al. 2001. Nature. 410:50-6. Gelmini S, et al. 2008. J Endocrinol Invest. 31:809-19. Zlotnik A. 2008. J Pathol. 215:211-3. |
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| Gene ID | P48061 |
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| UniProt | View information about CXCL12 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Stromal cell-derived factor 1, chemokine (C-X-C motif) ligand 12, SCYB12, PBSF, SCYB12, Pre-B Cell Growth-Stimulating Factor, Chemokine (C-X-C Motif) Ligand 12, Chemokine |
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