Biolegend, 598712, AZDye 647 Recombinant Human CCL21
10microg
Catalog Number: 598712
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| Source | Human CCL21, amino acids Ser24-Pro134 (Accession # O00585) with a C-terminal AZDye647, was expressed in E. coli. |
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| Molecular Mass | The 111 amino acid recombinant protein (Ser24-Pro134) has a predicted molecular weight of 14.2 kDa. |
| Purity | > 95%, as determined by mass spectrometry |
| Formulation | Lyophilized from 0.2 µm filtered solution of protein in DI-water. |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | Lyophilized, 10 µg per vial |
| Storage & Handling | Lyophilized at -20°C to -80°C until expirationLyophilized at 4°C for 6 monthsLyophilized at Room Temperature 1 monthReconstituted at -20°C to -80°C for 12 monthsReconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared at concentrations of 250 µg/mL up to 1 mg/mL in 0.22 µm filtered DI-water. Avoid repeated freeze/thaw cycles. |
| Activity | Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR7. The ED50 for this effect was measured at 0.5 to 3 µg/mL. |
| Application | FC, Live cell imaging |
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| Application Notes |
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| Protein has been verified for bioactivity prior to conjugation |
| Structure | CCL21 is a 111 amino acids molecule with a predicted molecular weight of 14.2 kDa. PDB ID – 2L4N. |
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| Distribution | CCL21 is highly expressed in HEV of lymph nodes and Peyer's patches. Also, it is expressed in the T cell zones of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL21 is expressed by endothelial cells in lymphatic venules |
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| Function | CCL21 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL21 and CCL19 chemoattract antigen-engaged B cells to the boundary of B and T zones to interact with T-helper cells. CCL19 and CCL21 inhibit apoptosis in DCs. |
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| Interaction | Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA) |
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| Ligand/Receptor | CCL21 binds to CCR7 receptor. |
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| Bioactivity | Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR7. The ED50 for this effect was measured at 0.5 to 3 µg/mL. |
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| Cell Type | Dendritic cells, Endothelial cells, Leukocytes, Lymphocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells |
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| Biology Area | Cell Biology, Immuno-Oncology, Immunology, Innate Immunity |
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| Molecular Family | Adaptor Proteins, Cytokines/Chemokines, Innate Immune Signaling |
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| Antigen References | Hedrick JA & Zlotnik A. 1997. J Immunol. 159:1589-93. Reif K, et al. 2002. Nature. 416:94-9. Ziegler E, et al. 2007. J Immunol. 179:6485-93. Pickens SR, et al. 2012. Arthritis Rheum. 64:2471-81. |
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| Gene ID | O00585 |
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| UniProt | View information about CCL21 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | 6CKine, Secondary lymphoid tissue chemokine (SLC), Exudos 2, Small inducible cytokine subfamily A, member 21 (SCYA21), SLC, TCA4, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 21, Chemokine (C-C Motif) Ligand 21, Chemokine |
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