Biolegend, 600755, Alexa Fluor® 647 anti-c-Fos Antibody
25microg
Catalog Number: 600755
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified recombinant human FOS protein fragments |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested ICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | For ICC we recommend PFA-MeoH fixation/permeabilization. We do not recommend methanol-only and PFA+ Triton X-100 fixation/permeabilization due to dim signal. For ICFC, we recommend fixation/permeabilization with the True-Phos™ Perm Buffer. Cytokine method was tried and failed due to no signal. |
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| RRID | AB_3097469 (BioLegend Cat. No. 600755) AB_3097469 (BioLegend Cat. No. 600756) |
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| Structure | c-Fos is a 330 amino acid protein with a predicted molecular weight of 41 kD. |
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| Distribution | Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes |
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| Function | FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation. |
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| Interaction | Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1 |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors |
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| Antigen References | Dony C and Gruss P. 1987. Nature. 328:711-4. Grigoriadis AE, et al. 1994. Science. 266:443-8. Glover JN and Harrison SC. 1995. Nature. 373:257-61. |
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| Gene ID | 2353 |
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| UniProt | View information about c-Fos on UniProt.org |
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| Clone | 7D6-A9-D4 |
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| Regulatory Status | RUO |
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| Other Names | Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos |
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| Isotype | Mouse IgG1, κ |
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