Biolegend, 600854, Alexa Fluor® 647 anti-FosB Antibody
100tests
Catalog Number: 600854
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Full-length recombinant FosB protein |
| Formulation | µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested |
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| Recommended Usage |
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| Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Methanol only and PFA + Methanol fix/perm methods were tested and produced inferior signal. ICFC: Cells were fixed and permeabilizes using True Phos Buffer system ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100 |
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| RRID | AB_2941600 (BioLegend Cat. No. 600853) AB_2941600 (BioLegend Cat. No. 600856) AB_2941600 (BioLegend Cat. No. 600854) |
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| Structure | FosB is a 338 amino acid protein with a predicted molecular weight of 36kD. |
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| Distribution | FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue. |
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| Function | FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation. |
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| Interaction | Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G/C TCA) SWI/SNF chromatin remodeling complex, DPP4 |
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| Cell Type | Mature Neurons |
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| Biology Area | Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors |
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| Molecular Family | Tumor Suppressors |
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| Antigen References | Nestler E. 2015. Eur J Pharmacol. 753:66-72. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61. |
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| Gene ID | 2354 |
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| UniProt | View information about FosB on UniProt.org |
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| Clone | W19011B |
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| Regulatory Status | RUO |
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| Other Names | G0/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B |
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| Isotype | Rat IgG2a, κ |
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