Biolegend, 601605, Alexa Fluor® 488 anti-human Cytokeratin 7 Antibody
25microg
Catalog Number: 601605
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Human KRT7 peptide (446-462) conjugated to KLH. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml (1:100-1:200 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Blue Laser (488 nm) |
| Application Notes | This antibody does not react with mouse (in-house tested). Additional reported applications (for the relevant formats) include: spatial biology (IBEX)1,2. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| RRID | AB_2728458 (BioLegend Cat. No. 601605) AB_2728459 (BioLegend Cat. No. 601606) |
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| Structure | Type II cytokeratin. 469 amino acids with a predicted molecular weight of 51 kD. |
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| Distribution | Cytoplasm. |
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| Function | KRT7 is a type II keratin, involved in the translational regulation of the human papillomavirus type 16 E7 mRNA. |
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| Interaction | KRT7 interacts with EIF3S10, HPV16 E7, and GPER1. |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Intermediate Filaments |
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| Antigen References | 1. Tajima Y, et al. 2017. Sci Rep. 7:40684. 2. Huang B, et al. 2016. Oncogene. 35:4927. 3. Szponar A, et al. 2012. Virchows Arch. 460:423. 4. Sano M, et al. 2010. Int J Oncol. 36:321. |
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| Gene ID | 3855 |
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| UniProt | View information about Cytokeratin 7 on UniProt.org |
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| Clone | W16155A |
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| Regulatory Status | RUO |
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| Other Names | Keratin type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Type-II keratin Kb7, Sarcolectin, SCL, Cytokeratin 7, Keratin 7 |
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| Isotype | Rat IgG2a, κ |
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