Biolegend, 606762, Alexa Fluor® 647 anti-Avi-tag Antibody
100microg
Catalog Number: 606762
Send an RFQ.
Category
Loading…
| Verified Reactivity | Epitope tag |
|---|---|
| Reported Reactivity | Species independent |
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Formulation | µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Recommended Usage |
|---|
| Each lot of the test-sized antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status. |
|---|
| Additional Product Notes | ICFC: Cells were fixed and permeabilizes using Cyto -Fast™ Buffer system ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100 |
|---|
| RRID | AB_3068042 (BioLegend Cat. No. 606759) AB_3068042 (BioLegend Cat. No. 606762) AB_3068042 (BioLegend Cat. No. 606760) |
|---|
| Structure | GLNDIFEAQKIEWHE. Avi-tag amino acid sequence |
|---|
| Distribution | Depends on the subcellular location of the tagged protein |
|---|
| Biology Area | Cell Biology, Protein Purification, Protein Trafficking and Clearance |
|---|
| Gene ID | NA |
|---|
| UniProt | View information about Avi-tag on UniProt.org |
|---|
| Clone | W19258A |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Avi-Tag, AviTag, Avi epitope Tag |
|---|
| Isotype | Rat IgG2b, κ |
|---|
