Biolegend, 607905, Alexa Fluor® 488 anti-GAPDH Antibody
25microg
Catalog Number: 607905
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant protein (161-335 a.a.) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
| Application Notes | This clone weakly reacts with rat GAPDH. |
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| RRID |
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| AB_2734506 (BioLegend Cat. No. 607905) AB_2734507 (BioLegend Cat. No. 607906) |
| Structure | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains. |
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| Distribution | Ubiquitously expressed, locate in cytoplasm and perinuclear region. |
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| Function | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains. |
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| Interaction | Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2. |
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| Biology Area | Cell Biology |
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| Antigen References | Ercolani L, et al. 1988. J. Biol. Chem. 263:15335. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527. Zheng L, et al. 2003. Cell. 114:255. Wang Q, et al. 2005. FASEB J. 19:869. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405. |
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| Gene ID | 5590 |
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| UniProt | View information about GAPDH on UniProt.org |
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| Clone | W17079A |
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| Regulatory Status | RUO |
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| Other Names | glyceraldehyde-3-phosphate dehydrogenase |
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| Isotype | Rat IgG2a, κ |
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