Biolegend, 608455, Alexa Fluor® 488 anti-RBFOX3 (NeuN) Antibody
25microg
Catalog Number: 608455
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Full length recombinant human RBFOX3, isoform 5 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality tested IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry on frozen tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunohistochemistry in paraffin-embedded tissues, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Additional Product Notes | For immunohistochemistry in paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) results in dim signal. |
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| RRID | AB_3106162 (BioLegend Cat. No. 608455) AB_3106162 (BioLegend Cat. No. 608456) |
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| Structure | RBFox3 is a 312 amino acid protein with a predicted molecular weight of 34 kD |
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| Distribution | Mature Neurons, Neural Stem Cells/ Cytoplasm and Nucleus |
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| Function | Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Cell Type | Mature Neurons, Neural Stem Cells |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | Nuclear Markers |
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| Antigen References | Dhekne HS, et al. 2018. Elife. 7:e40202. Sun Y, et al. 2020. J Neuroinflammation. 17:72. Schwabenland M, et al. 2021. Immunity. 54:1594-1610. |
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| Gene ID | 146713 |
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| UniProt | View information about RBFOX3 on UniProt.org |
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| Clone | W18230A |
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| Regulatory Status | RUO |
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| Other Names | NeuN, RNA binding protein fox-1 homolog 3 (RBFox3), hexaribonucleotide binding protein 3, HRNBP3 |
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| Isotype | Rat IgG2a, κ |
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