Biolegend, 611010, Alexa Fluor® 647 anti-HDAC1 Phospho (Ser406) Antibody
100tests
Catalog Number: 611010
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| Verified Reactivity | Human, Zebrafish |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site. This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062. Clone BT-15 cross-reacts to Zebrafish2 |
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| Application References(PubMed link indicates BioLegend citation) | Segre CV, et al, 2016. mAbs. 8: 37-42 Loponte S, et al, 2016. Sci Rep, 6: 30213. |
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| RRID | AB_2910487 (BioLegend Cat. No. 611009) AB_2910487 (BioLegend Cat. No. 611010) |
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| Structure | HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD. |
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| Distribution | Nucleus/Ubiquitously expressed |
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| Function | Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme. |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics |
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| Molecular Family | Nuclear Markers, Phospho-Proteins |
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| Antigen References | Segre CV, et al, 2016. mAbs. 8: 37-42. Loponte S, et al, 2016. Sci Rep, 6: 30213. |
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| Gene ID | 3065 |
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| UniProt | View information about HDAC1 Phospho Ser406 on UniProt.org |
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| Clone | BT-15 |
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| Regulatory Status | RUO |
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| Other Names | Histone Deactylase 1, RPD3L1 |
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| Isotype | Mouse IgG2b, κ |
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