Biolegend, 612003, Alexa Fluor® 647 anti-FOXA1 Antibody
25microg
Catalog Number: 612003
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Full length recombinant human FOXA1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| When using this clone for western blot, we highly recommend using lysate that has not been subjected to any freeze-thaw cycles. |
| Additional Product Notes | NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. |
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| Application References(PubMed link indicates BioLegend citation) | Besenard V, et al. 2004. Gene Expr Patterns. 5:193-208. (IHC-P) |
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| RRID | AB_2801117 (BioLegend Cat. No. 612003) AB_2801118 (BioLegend Cat. No. 612004) |
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| Structure | FOXA1 is a 472 amino acid protein with a predicted molecular weight of 49 kD. |
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| Distribution | Nucleus/Early endoderm derived cells |
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| Function | Transcriptional activator |
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| Interaction | FOXA2, NKX2-1, Histones H3 and H4 |
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| Biology Area | Cell Biology, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Gene ID | 3169 |
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| UniProt | View information about FOXA1 on UniProt.org |
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| Clone | 2F83 |
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| Regulatory Status | RUO |
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| Other Names | Forkhead box protein A1, HNF3α, Hepatic nuclear factor 3 α |
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| Isotype | Mouse IgG1, κ |
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