Biolegend, 614306, Alexa Fluor® 594 anti-PARP Antibody
100microg
Catalog Number: 614306
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant (partial), N-terminal 2/3 sequence of PARP |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality testedIHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes | This antibody recognizes N-terminal short cleaved PARP after Staurosporine treatment at around 24kDa. |
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| Application References(PubMed link indicates BioLegend citation) | Bogiatzi SI, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed. Gu Y, et al. 2013. Acta Biochim Biophys Sin. PubMed |
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| RRID | AB_2563430 (BioLegend Cat. No. 614306) |
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| Structure | PARP family, BRCT domain, homo- or hetero-dimer; 113 kD |
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| Distribution | Nuclear |
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| Function | Molecular "nick sensor"; base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism; protein modification may enhance or repress transcription |
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| Interaction | Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others |
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| Modification | Ribosylation by PARP2 |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Cherney B, et al. 1987. P. Natl. Acad. Sci. USA 84:8370. 2. Ikejima M, et al. 1990. J. Biol. Chem. 265:21907. 3. Ying W, et al. 2001. P. Natl. Acad. Sci. USA 98:12227. 4. Noel G, et al. 2003. BMC Cell Biol. 4:7. |
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| Regulation | Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins |
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| Gene ID | 142 |
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| UniProt | View information about PARP on UniProt.org |
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| Clone | 5A5 |
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| Regulatory Status | RUO |
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| Other Names | Poly (ADP-ribose) polymerase |
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| Isotype | Mouse IgG1, κ |
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