Biolegend, 614853, Alexa Fluor® 488 anti-α Smooth Muscle Actin Antibody
25microg
Catalog Number: 614853
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH). |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality testedICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | For use in immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissues, we recommend heat-induced antigen retrieval with either Tris-EDTA pH 9.0 or Citrate Buffer, 10X (Cat. No. 420902).For use in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X.Previously Covance catalog# MMS-466S. |
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| RRID | AB_3662308 (BioLegend Cat. No. 614853) AB_3662308 (BioLegend Cat. No. 614854) |
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| Structure | α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD. |
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| Distribution | Cytoplasmic, cytoskeleton |
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| Function | Cell motility |
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| Interaction | α smooth muscle actins bind to myosins to produce motive force |
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| Cell Type | Mesenchymal Stem Cells, Monocytes |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Microfilaments |
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| Antigen References | Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83. Rizeq MN, et al. 1994. Hum Pathol. 25:671-7. Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42 Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74. Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613. Skalli O, et al. 1986. J Cell Biol. 103:2787-96. |
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| Gene ID | 59 |
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| UniProt | View information about alpha Smooth Muscle Actin on UniProt.org |
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| Clone | 1A4 |
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| Regulatory Status | RUO |
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| Other Names | Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein |
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| Isotype | Mouse IgG2a, κ |
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