Biolegend, 614862, Alexa Fluor® 594 anti-α Smooth Muscle Actin Antibody
100microg
Catalog Number: 614862
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human, Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH). |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested ICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Additional Product Notes | For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -100% ice-cold methanol only -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol |
|---|
| Structure | α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD. |
|---|
| Distribution | Cytoplasmic, cytoskeleton |
|---|
| Function | Cell motility |
|---|
| Interaction | α smooth muscle actins bind to myosins to produce motive force |
|---|
| Cell Type | Mesenchymal Stem Cells, Monocytes |
|---|
| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers |
|---|
| Molecular Family | Microfilaments |
|---|
| Antigen References | Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83. Rizeq MN, et al. 1994. Hum Pathol. 25:671-7. Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42 Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74. Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613. Skalli O, et al. 1986. J Cell Biol. 103:2787-96. |
|---|
| Gene ID | 59 |
|---|
| UniProt | View information about alpha Smooth Muscle Actin on UniProt.org |
|---|
| Clone | 1A4 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein |
|---|
| Isotype | Mouse IgG2a, κ |
|---|
