Biolegend, 619306, Alexa Fluor® 488 anti-TIF1β (KAP-1, TRIM28) Antibody
100microg
Catalog Number: 619306
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). This clone may produce increased background by western blot when used at concentrations exceeding 0.1 µg/mL. |
| Application References(PubMed link indicates BioLegend citation) | Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP) |
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| RRID | AB_2566440 (BioLegend Cat. No. 619306) |
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| Structure | Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD |
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| Distribution | Nuclear, associates with specific chromatin regions |
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| Function | Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression |
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| Biology Area | Cell Biology, Immunology, Transcription Factors |
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| Molecular Family | Protein Kinases/Phosphatase |
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| Antigen References | 1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366. 2. Schultz DC, et al. 2002. Genes Dev. 16:919. 3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859. 4. Friedman JR, et al. 1996. Genes Dev. 10:2067. 5. Messerschmidt DM, et al. 2012. Science 335:1499. 6. Chikuma S, et al. 2012. Nat. Immunol. 13:596. |
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| Gene ID | 10155 |
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| UniProt | View information about TIF1beta on UniProt.org |
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| Clone | 20A1 |
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| Regulatory Status | RUO |
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| Other Names | Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1 |
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| Isotype | Mouse IgG1, κ |
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