Biolegend, 622403, Purified anti-p38 MAPK Antibody
50microl
Catalog Number: 622403
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| Verified Reactivity | Mouse, Rat, Human |
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| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Immunogen | peptide-KLH, C terminal |
| Formulation | PBS containing 0.09% sodium azide and 0.2% gelatin.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | WB - Quality tested IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:4000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:200 is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| This product may contain other non-IgG subtypes. |
| Application References(PubMed link indicates BioLegend citation) | da Silva, et al. 2011. Biochim Biophys Acta. 181:1863. PubMed Chen W, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:279. PubMed Maeda S, et al. 2014. J Biol Chem. 289:23786. PubMed |
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| Product Citations | Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed Jiang Q, et al. 2022. Stem Cells Int. 2022:5897194. PubMed |
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| RRID | AB_2876740 (BioLegend Cat. No. 622403) |
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| Structure | Belongs to serine/threonine kinase family, MAP subfamily, 38 kD |
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| Distribution | Ubiquitously expressed, nuclear and cytoplasmic |
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| Function | p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock-out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2, MEF2C and MAX. |
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| Interaction | Interacts with MAX, Cdc25B, Cdc25C. Binds to kinase interaction domain in the protein tyrosine phosphatase PTPRR; this interaction retains p38 MAPK in the cytoplasm. |
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| Modification | Phosphorylation |
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| Biology Area | Cell Biology |
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| Molecular Family | Protein Kinases/Phosphatase |
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| Antigen References | 1. Bulavin DV, et al. 2001. Nature 411:102. 2. Han J, et al. 1994. Science 265:808. 3. Lee JC, et al. 1994. Nature 372:739. |
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| Regulation | Activated by environmental stress, many pro-inflammatory cytokines and lipopolysaccharide. Dual phosphorylation by MAP2K3 and MAP2K6 is required for activation of p38 MAPK. |
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| Gene ID | 80824 |
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| UniProt | View information about p38 MAPK on UniProt.org |
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| Clone | Poly6224 |
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| Regulatory Status | RUO |
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| Other Names | Stress activated kinase p38, p38 MAP kinase, stress activated protein kinase 2A |
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| Isotype | Rabbit Polyclonal IgG |
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