Biolegend, 623405, Alexa Fluor® 647 anti-Acetylated Lysine Antibody
25tests
Catalog Number: 623405
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| Verified Reactivity | Species independent |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Acetylated protein mixture |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed |
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| Product Citations | Martins VF, et al. 2019. Am J Physiol Cell Physiol. 317:C964. PubMed Martins VF, et al. 2021. JCI Insight. Online ahead of print. PubMed Masuda N, et al. 2021. BMC Cancer. 21:1269. PubMed |
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| RRID | AB_2565505 (BioLegend Cat. No. 623405) AB_2565506 (BioLegend Cat. No. 623406) |
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| Structure | Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine |
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| Function | Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions |
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| Biology Area | Cell Biology, Chromatin Remodeling/Epigenetics |
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| Antigen References | 1. Wang S, et al. 2004. Novartis Found. Symp. 259:238. 2. Legube G, et al. 2003. EMBO Rep. 4:944. 3. Chen LF, et al. 2003. J. Mol. Med. 81:549. |
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| Gene ID | NA |
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| Clone | 15G10 |
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| Regulatory Status | RUO |
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| Isotype | Mouse IgG2b, κ |
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