Biolegend, 626809, Alexa Fluor® 647 anti-c-Myc Antibody
25microg
Catalog Number: 626809
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| Verified Reactivity | Epitope tag |
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| Reported Reactivity | Species independent |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Amino acids 408-439, C-terminal region of human c-myc |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Red Laser (633 nm) |
| Application Notes | *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody. Reactivity with endogenous myc in applications other than IHC-P (ICC, WB, etc.) is not verified. |
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| Application References(PubMed link indicates BioLegend citation) | Rao H, et al. 2001. Nature. 410:955. Peters CS, et al. 2001. J Biol Chem. 276:13718. Munro S, Pelham HR. 1987. Cell. 48:899. Evan GI, et al. 1985. Mol Cell Biol. 5:3610. |
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| Product Citations | Merljak E, et al. 2023. Nat Commun. 14:1995. PubMed Gioulbasani M, et al. 2024. Commun Biol. 1606:7. PubMed |
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| RRID | AB_2888732 (BioLegend Cat. No. 626809) AB_2888732 (BioLegend Cat. No. 626810) |
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| Biology Area | Cell Biology, Protein Purification, Protein Synthesis |
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| Antigen References | Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217. Battey J, et al. 1983. Cell 34:779. Beimling P, et al. 1985. Biochemistry 24:6349. |
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| Gene ID | 4609 |
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| UniProt | View information about c-Myc on UniProt.org |
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| Clone | 9E10 |
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| Regulatory Status | RUO |
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| Other Names | myc tag, c-Myc tag, cMyc tag, Myc epitope tag |
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| Isotype | Mouse IgG1, κ |
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