Biolegend, 627906, Alexa Fluor® 488 anti-Tubulin-α Antibody
100microg
Catalog Number: 627906
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Category
| Verified Reactivity | Human, Mouse, Rat, All Species |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant full length human α-tubulin |
| Formulation | This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistrys, a concentration range of 1.0 - 4.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Chou HYE, et al. 2006. J. Biol. Chem. 281:15201. |
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| Product Citations | Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed Khalin I, et al. 2020. ACS Nano. 14:9755. PubMed |
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| RRID | AB_893643 (BioLegend Cat. No. 627905) AB_893642 (BioLegend Cat. No. 627906) |
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| Structure | Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD. |
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| Distribution | Ubiquitous |
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| Function | Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site |
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| Interaction | RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A |
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| Modification | Phosphorylation, acetylation |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Microtubules |
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| Antigen References | 1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738. 2. Miller FD, et al. 1987. J. Cell Biol. 105:3065. 3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96. |
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| Gene ID | 84790 |
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| UniProt | View information about Tubulin-alpha on UniProt.org |
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| Clone | 10D8 |
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| Regulatory Status | RUO |
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| Other Names | α-tubulin, Tubulin, alpha-ubiquitous chain |
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| Isotype | Mouse IgM, κ |
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