Biolegend, 628604, Alexa Fluor® 647 anti-Cytokeratin (pan reactive) Antibody
100microg
Catalog Number: 628604
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Keratin enriched fraction from human epidermoid carcinoma. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested ICFC - Verified SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Kovarik J. 1988. Int. J. Cancer Suppl. 3:50. Bartek J, et al. 1991. J. Pathol. 164:215. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed Comito G, et al. 2012. Cancer Lett. 324:31. PubMed Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow) Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Roy S, et al. 2019. J Cell Biochem. 120:4804. PubMed Hendricks A, et al. 2021. Front Oncol. 11:646885. PubMed Hendricks A, et al. 2020. Cancers (Basel). 12:00. PubMed Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed |
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| RRID | AB_2563652 (BioLegend Cat. No. 628604) |
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| Structure | Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits |
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| Distribution | Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus |
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| Function | Intermediate filament protein involved with the cytoskeleton. |
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| Interaction | Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Intermediate Filaments |
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| Gene ID | 3875 |
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| UniProt | View information about Cytokeratin pan reactive on UniProt.org |
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| Clone | C-11 |
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| Regulatory Status | RUO |
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| Other Names | Cytokeratin, Keratin |
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| Isotype | Mouse IgG1, κ |
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